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Description
Hi Struo Team,
Thank you for creating and maintaining these valuable databases for the community.
I was wondering if you could kindly point me to where I can download the file mpa_v30_CHOCOPhlAn_201901_marker_info.txt as you indicated in below usage?
I have downloaded uniref90 from your release which only contains below files:
all_genes_annot.1.bt2l all_genes_annot.3.bt2l all_genes_annot.rev.1.bt2l genome_reps_filt_annot.fna.gz
all_genes_annot.2.bt2l all_genes_annot.4.bt2l all_genes_annot.rev.2.bt2l genome_reps_filt_annot.faa.gz genome_reps_filt_annot.tsv.gz
uniref90_201901b.dmnd
Thank you so much for your help!
Ivy
Example of a HUMANnN3 run
Run HUMAnN3 with custom databases created by Struo2. Change that PATHs as necessary.STRUO2_OUT_DIR=./struo2_output/
NUC_DB=$STRUO2_OUT_DIR/humann3/uniref50/genome_reps_filt_annot.fna.gz
PROT_DB=$STRUO2_OUT_DIR/humann3/uniref50/protein_database/uniref50_201901.dmnd
> MP_DB=$STRUO2_OUT_DIR/mpa_v30_CHOCOPhlAn_201901_marker_info.txtREADS=/path/to/example/read/files/CHANGE/THIS/reads.fq
metaphlan database not actually used
touch $MP_DB
humann3 run
humann3 --bypass-nucleotide-index
--nucleotide-database $NUC_DB
--protein-database $PROT_DB
--metaphlan-options "--bowtie2db $MP_DB"
--output-basename humann
--input $READS
--output humann_output