Commit 2a6ec42
Update pipeline document (#136)
* updated pipeline document
- standardized bismark output naming in parameter/output definitions
- added missing parameter definitions for all positional arguments
- added note about GeneLab suffix difference for DNA and RNA methylation
- removed bold formatting from files that are not output (trimming
reports and gene-to-transcript map)
- trimming report data is included in trimming multiqc report
- gene-to-transcript map is part of reference generation and not study
specific
- renamed deduplicated sorted output, alignment and bismark multiqc report output
- reconfigured MethylKit section to conform with output able format for other pipelines
- Combined all pair-wise differential methylation analyses into one output table for
bases and one output table for tiles (without applying any significance cutoffs so data
from all regions are available), rather than providing multiple different files and only
providing differential methylation data for regions that meet significance cutoffs.
- added normalization step prior to differential methylation calculation
- replaced write.csv with data.table::fwrite for differential methylation output
tables for better write efficiency when these tables are large.
- reconfigure workflow_code folder
- added isa_to_runsheet plugins folder
- added runsheet spec
- add runsheet generation step
- add definition for dist.to.feature
- switch to using species instead of name column in annotations.csv
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Co-authored-by: asaravia-butler <70983120+asaravia-butler@users.noreply.github.com>1 parent 810fc7a commit 2a6ec42
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- Pipeline_GL-DPPD-7113_Versions
- Workflow_Documentation
- isa_to_runsheet_plugins
- dp_tools__methylseq_dna
- dp_tools__methylseq_rna
- runsheet_specification
- paired_end_runsheet
- single_end_runsheet
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