Would circulator change the assembly? #189
Description
I have a genome assembly that was assembled carefully with Trycycler that was only one contig and yet uncircularized. Trying to circularize it with circlator, and annotating it and I found the result slightly changed.
Pre-circularization:
Genome length 2948785
CDs:2733
hypotheticals:141
Post-circularization:
Genome length 2948782
CDs:2732
hypotheticals:142
The following is my command:
circlator all --merge_min_id 85 --merge_breaklen 1000 --threads 10 cas_124370.fasta cas124370.fastq cir_cas_124370
Why is there a subtle change on this? should I be concerned about the change on the assembly? I hope the slight changes is only mergning the ends but not changing anything else, since I used a really robust pipeline to assemble the genome using Trycycler and trust it more.