Use of Analyst with isotopic MS1 quant data?

[BjoernZiehr]

Hello. Is there a way that I can use Analyst with MS1 quant (heavy vs light) data? My workflow was essentially like the SILAC demo, but with custom mass modifications. I am comparing heavy vs light modified peptides.

The combined_protein_label_quant.tsv contains HvsL log ratios which is good, but not much else.

Example:

Protein contam_sp|P00883|ALDOA_RABIT
Protein ID P00883
Entry Name ALDOA_RABIT
Gene ALDOA
Protein Description Fructose-bisphosphate aldolase A
HvL_1 Light Intensity 3.24E+08
HvL_2 Light Intensity 3.24E+08
HvL_3 Light Intensity 3.24E+08
HvL_1 Heavy Intensity 3.81E+08
HvL_2 Heavy Intensity 3.81E+08
HvL_3 Heavy Intensity 3.81E+08
HvL_1 Ratios HL 6
HvL_2 Ratios HL 6
HvL_3 Ratios HL 6
HvL_1 Median Log2 Ratios HL -0.163571126
HvL_2 Median Log2 Ratios HL -0.163571126
HvL_3 Median Log2 Ratios HL -0.163571126

[hsiaoyi0504]

@BjoernZiehr Currently it's not supported, but if you share the example data (yihsiao@umich.edu) you have with me, I can take a look and see if we could support. Thanks.