POMT Manuscript Code Archive

This github repository describes the code and data sources used to generate the figures described in the following manuscript: https://www.biorxiv.org/content/10.1101/2025.08.18.670743v1

Data Sources

RNA-Seq

Bulk RNA-Seq from human pLGG tumors can be accessed through [this: PMID] manuscript Bulk RNA-Seq from IUE tumor models is deposted at [this: GEO]

scRNA-Seq

Single cell RNA-Seq data from human pLGG tumors is deposited at [this: GEO]

Running scripts

Infer KIAA1549 topology and transmembrane domain

python /pomt_manuscript_code/fusion_partner_analysis/infer_kiaa1549_tm_domain.py

Cellprofiler image analysis scripts

Scripts necessary for running cellprofiler to analyze KIAA1549::BRAF localization are in the cell_profiler subdirectory

Analysis of number of unique fusion species in pediatric and adult tumors

Analysis of scRNA-Seq data

Analysis of POLR2B chronos score

Analysis of IUE RNA-Seq

Analysis of BRAF fusion breakpoints

Generating oncoprint

Predicting myristoylation motif or transmembrane domain

Analysis of screen results

Analaysis of DepMap scores for POMT and DPM complex protein