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Sequence-manipulation

gc_data.plx
This is a script for calculating the GC content of settled length around target site at a box length continuously (from the first base to the last base of the settled region with the box length as counting unit);

  • desired site infomation file contains chromosome and site infomation in the first and second column,no headings;
  • desired sequence file is in .fa format, with chromosome id after >;
  • desired region should be smaller than the length of the sequence;
  • GC box length is the length of the region for calculating GC content;
  • base 'N' is excluded from calculation.

seq_extract.plx
This is a script for fetching the sequence of settled length around target site from fasta file;

  • desired site infomation file contains identifier and site infomation in the first and second column,no headings;
  • desired sequence file is in .fa format, with identifier after >;
  • desired region should be smaller than the length of the sequences.

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